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Fig. 2 | BMC Genetics

Fig. 2

From: Generation of megabase-scale deletions, inversions and duplications involving the Contactin-6 gene in mice by CRISPR/Cas9 technology

Fig. 2

Results of genotyping of 11 offspring F0 by PCR for detection of carriers: deletion of the Cntn6 gene (panel a), its duplications (panel b), its inversions sequenced right and left sides (panel c and d, respectively). Primer pairs used for detection of deletions were FWD1.1 + REV2, duplications were FWD2.1 + REV1 and inversions were REV1 + REV2 for right side, and FWD1.1 + FWD2.2 for left side. Expected size of PRC products: deletion 636 bp, duplication 349 bp, inversion right side 790 bp, inversion left side 323 bp

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